cacl2 heat shock method
CalciumChlorideimpartsapositivechargetothecellwall.SinceDNAisnegativelychargedmolecule,thepositivechargeonthecellwallimpartedbycalcium ...,Heatshockthecellsina42oCwaterbathfor45to60secondsandreturntoicefor1minute.Add1.2mlofLBbrothtothetube,mixand...
Preparation of calcium competent Escherichia coli and ...
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由AYChang著作·被引用189次—Calciumchlorideheat-shocktransformationisapowerfulmolecularbiologytechniqueusedtointroduceforeignDNAintoahostcell.
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